Expression and significance of monocyte chemotactic protein in the pathogenesis of ankylosing spondylitis
- VernacularTitle:单核细胞趋化蛋白在强直性脊性炎患者中的表达与意义
- Author:
Feng HUANG
;
Jieruo GU
;
Jianglin ZHANG
;
Wei ZHAO
;
Jian ZHU
;
Tianwang LI
;
Yuqiong WU
;
Deen YU
- Publication Type:Journal Article
- Keywords:
Spondylitis,ankyolsing;
cDNA microarray;
Monocyte chemotactic protein
- From:
Chinese Journal of Rheumatology
2001;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective Previous study had shown that signal transduction was changed when Hela cells were transfected with HLA-B27gene.An increase of monocyte chemotactic protein-1(MCP-1)of Hela cells was observed.To determine the role of MCP in the pathogenesis of ankylosing spondylitis(AS),expression of MCP-1,2,3and4in peripheral blood mononuclear cells(PBMC),synovial fluid mononuclear cells(SFMC)and synovial tis-sues of AS patients were tested.Methods Gene expression profiles of PBMC from AS patients and healthy volun-teers were determined by cDNA microarray with1176target gene filter.The differentiated expressed gene MCP-1in PBMC,SFMC and synovial tissue of AS patients were confirmed by semi-quantitative RT-PCR.Results The gene expression profile of SFMC of AS patients was significantly different from those of PBMC from AS and PBMC from healthy volunteers.The MCP-1level was positively correlated with MCP-3(r=0.76,P=0.03).The expressions of MCP-1were higher in synovial tissues of AS than those of healthy volunteers(P=0.0035).MCP-1levels in monocytes of AS patients and control subjects were increased after LPS stimulation for4hours.Conclusions There is increased expression of MCP-1in SFMC and synovial tissue of AS patients.The results indicate that MCP-1may play a potential key role in the homing of cells migrating from blood to joint and in the pathogenesis of joint inflammation in AS patients.
