Effect of Storing Time of Venous Blood Samples on the Differential Counts of White Blood Cells by Sysmex XE-2100 Hematology Analyzer
- VernacularTitle:静脉血放置时间对 Sysmex XE-2100血液分析仪白细胞分类结果的影响
- Author:
Weizhen FANG
;
Mei CHEN
;
Yuru FU
;
Changzhen XU
- Publication Type:Journal Article
- Keywords:
venous blood;
Sysmex XE-2100;
time;
differential count of WBC
- From:
Journal of Tropical Medicine
2005;5(6):786-789,799
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the influence of the storing time of venous blood samples on the differential count of WBC by Sysmex XE-2100 hematology analyzer. Methods At room temperature, the precision of the differential count of WBC by Sysmex XE-2100 hematology analyzer were tested. 38 samples were taken the differential count of WBC by Sysmex XE-2100 hematology analyzer after stored for 0, 2, 4, 8, 24 and 48 h. Differential count of WBC was also taken under microscope for comparison. Results The precision of differential count of WBC by Sysmex XE-2100for all the samples was in the allowable range. The correlation coefficient of the differential count of WBC by two methods for neutrophils, lymphocytes, monocytes, eosinophils and basophils were 0.9859, 0.9775, 0.8053, 0.8695and 0.5243 (P<0.01). There was insignificant difference in the test at 8h, very significant difference of MONO and EOS at 48 h. Differences of EOS, MONO between two methods were significant increased at 8 h. Conclusion At room temperature, the differential count of WBC of venous blood samples by Sysmex XE-2100 hematology analyzer should finish within 8 h.