Determination of 2?-hydroxy-ursolic acid from Herba Rabdosiae Serrae in various collecting periods
- VernacularTitle:不同采收期溪黄草中2?-羟基熊果酸含量的动态研究
- Author:
Jianfeng WU
;
Bin LIU
;
Chenchen ZHU
;
Xiaopin LAI
- Publication Type:Journal Article
- Keywords:
Herba Rabdosia Serrae;
collecting periods;
HPLC;
2?-hydroxy-ursolic acid
- From:
Chinese Traditional and Herbal Drugs
1994;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Object To supply the basis for the optimal collecting period for Herba Rabdosiae Serrae (HRS). To lay down relevant Standard Operating practice (SOP) in accordance with GAP of Chinese medicinal materials and put them into practice with providing basic researching data. Methods To determine the contents of 2?-hydroxy-ursolic acid in HRS by various collecting periods (cultured under GAP) by RP-HPLC. Chromatographic conditions: Kromasil RP-C_ 18 (250 mm ? 4.6 mm,5 ?m) column;the mobile phase was acetonitrile-0.05% trifluoro-acetic acid solution (70.5∶29.5);the velocity of flow was 0.8 mL/min;column temperature was 25 ℃;and the detection wavelength was at 210 nm. Results In various collecting periods,contents of 2?-hydroxy-ursolic acid in HRS are the highest in the collection of earlier flower of Rabdosia Lophanthoides var. gerardianus (Beutham) H. Hara in August. Conclusion It suggests that HRS be gathered in the leaves luxuriance before blooming.
