Vascular endothelial growth factor_(165) can reduce the cerebral lesion caused by focal cerebral ischemia in rats
- VernacularTitle:血管内皮生长因子_(165)对局灶性脑梗死的影响
- Author:
Yanqun CHANG
;
Yong LIU
;
Huayan FAN
- Publication Type:Journal Article
- Keywords:
Cerebral infarct;
Vascular endothelial g rowth factor 165;
Gene therapy
- From:
Chinese Journal of Physical Medicine and Rehabilitation
2004;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of naked DN A encoding vascular endothelial growth factor 165 (VEGF 165 )on cerebra l infarction in rats. Methods Following establishme nt of a permanent middle cerebral artery occlusion (MACO) model by nylon suture embolization in Wistar rats, the puCCAGGS/hVEGF 165 was directly injected i nto the ischemic tissues through skull hole. Seven days later, the rats were sac rificed. The infarct volume was measured by 2% TTC staining technique, then the expression of VEGF 165 gene and vascular proliferation were measured by use of RT-PCR and immumohistochemistry methods. Results Expression of VEGF 165 mRNA and VEGF protein in the therapy group increas ed. Compared with the control group, the number of vessels of the therapy group was significantly higher (50.76/HPF vs 40.67/HPF)( P