Comparison of Monoamine Neurotransmitters in Two Morphine-dependent Rat Models
- VernacularTitle:两种吗啡依赖大鼠模型脑内单胺神经递质水平的比较
- Author:
Zhixian MO
;
Ye LIU
;
Jin CHEN
- Publication Type:Journal Article
- Keywords:
Morphine;
Physical dependence;
Psychic dependence;
Monoamine neurotransmitter;
Withdrawal syndrome;
Conditional place preference
- From:
Chinese Journal of Comparative Medicine
2009;19(3):6-9,24
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the changes of monoamine neurotransmitters of the brain in physical dependence induced by morphine in rats.Methods A physical dependent rat model was established with morphine in a gradually in creasing doses and the withdrawal syndrome was scored after naloxone precipitation.The conditioned place preference(CPP)in rats induced by morphine was used to investigate psychic dependence in rats.Contents of norepinephrine(NE0,dopamine(DA)and serotonin(5-HT)in hypothalamus of rats were assayed with a fluorescent method. Results (1)In naloxone-precipitated withdrawal test of morphine-dependent model rats,after morphine had been withdrawn,morphine-abstinent rats presented marked withdrawal symptoms and signs,their withdrawal scores were significantly increased,and the levels of NE and 5-HT in the rat brain were obviously enhanced,but the content of DA was reduced.(2)In CPP test,morphine caused a marked place preference in rats and the levels of DA and 5-HT in the rat brain were obviously enhanced,but the content of NE was reduced.Conclusiion Morphine dependence development and withdrawal are closely connected with monoamine neurotransmitters in CNS.In the physical dependent model induced by morphine in rats,the rising of NE and 5-HT in the rat brain were significant,but in psychic dependent model induced by morphine in rats,the levels of DA in the rat brain were enhanced p-redominately.
