Role of interferon-? on smooth muscle cells proliferation and migration after balloon injury
- VernacularTitle:干扰素-?对动脉损伤后平滑肌细胞增殖和迁移的作用
- Author:
Yu MEI
;
Guizhao WANG
;
Yonglin HUANG
- Publication Type:Journal Article
- Keywords:
Interferon ?;
Transforming growth factor ? 1;
Smooth muscle cell;
Matrix metalloproteinase 2;
Artery balloon injury
- From:
Journal of Interventional Radiology
2003;0(S1):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of interferon ?on smooth muscle cells proliferation and migration after balloon injury. Methods Animal model of rabbit iliac artery balloon injury was set up, smooth muscle cells derived from injured artery were cultured. Smooth muscle cells were divided into four groups ( control, TGF ? 1, IFN ?, TGF ? 1 and IFN ?).Cells from each group were treated with medium or TGF ? 1 (10 ng/ml) and/or IFN ?(500 u/ml) for 72 h separately. Smooth muscle cell proliferation was determined by cell count and MTT, migration of smooth muscle cells was also detected. Matrix metalloproteinase 2 was also detected by zymography. Results Our results showed that, compared with group control(2.875?0.323?10 5 cells/ml,279.9?8.129 ?m), TGF ? 1 increased cell count (4.188?0.239?10 5 cells/ml, P
