Genotype distributions of the 5 sites SNPs in the 3 subtypes of beta adrenoreceptor
- VernacularTitle:Beta肾上腺素能受体3种亚型基因的5位点SNP的基因型分布
- Author:
Zhengxiang XIE
- Publication Type:Journal Article
- Keywords:
Beta adrenoreceptor;
Single nucleotide polymorphisms;
Genotype distribution
- From:
Journal of Chongqing Medical University
2003;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the genotype-by site distributions of SNP(single nucleotide polymorphisms)at the 5 sites in the 3 subtypes of beta adrenoreceptor(Beta-AR or B-AR).Methods:DNA was extracted from the peripheral venous blood of 338 subjects using Blood DNA Mini Kit.The 5 sites SNP in the 3 subtypes of Beta-AR were genotyped by PCR-RFLP(polymerase chain reaction-restriction fragment length polymorphism) and allele specified PCR techniques.Results:The natural distribution characteristics for SNP at the 5 sites in the 3 subtypes of Beta-AR of 338 subjects were gotten.The predominant phenotypes are as follows:Beta1-AR wild homozygote Ser/Ser49(70%),Beta1-AR wild homozygote Arg/Arg389(63%),Beta2-AR heterozygote Arg/Gly16(80%),Beta2-AR heterozygote Gln/Glu27(50%),Beta3-AR wild homozygote Trp/Trp64(65%).Conclusion:It is heterogeneous in distribution in different styles for the genotype-by site distribution of SNP at the 5 sites in the 3 subtypes of Beta-AR,which is the basis for further investigating the relationship among the distribution,gene function and diseases.
