Improvement in orthotopic liver transplantation in rat and related operation skills
- VernacularTitle:改进的大鼠原位肝移植术与相关手术技巧
- Author:
Yong PENG
- Publication Type:Journal Article
- Keywords:
Rat;
Original liver transplantation;
Surgical operation skill
- From:
Journal of Chongqing Medical University
2003;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To improve orthotopic liver transplantation in the rat and conclude related operation skills.Methods:160 cases of orthotopic liver transplantation models in the rat were established by improved two-cuff technique.The anastomosis of portal vein between donor and recipient was performed by cuff technique.The same method was used to anastomose infrahepatic vena cava.The suprahepatic vena cava anastomosis was performed by suture and bile duct anastomosis was completed end to end by Teflon catheter.Results:The average time of donor operation,preparing graft and anhepatic period was 46min,24min and 21min respectively.The anastomosis time of suprahepatic vena cava,portal vein,infrahepatic vena cava, and bile duct was 13min,3min,4min,and 3min respectively.Main reasons for death during operation were pneumothorax,anesthesia and bleeding,and the successful rate of operation was 92.5%.Infection,obstruction of biliary tract and graft failure were attributed to the main causes for mortality after operation.Conclusion:Making orthotopic liver transplantation in the rat requires professional microsurgical techniques and careful operation.The improved two-cuff technique possesses advantages of short anhepatic phase,high operative successful rate,and is an ideal method for establishing this animal model.
