Determination of Indigo Content in Qingxin Sushitie by HPLC
- VernacularTitle:HPLC法测定青辛速释贴中靛蓝的含量
- Author:
Rongzhen PENG
;
Guoshen ZHENG
;
Guangliang LUO
- Publication Type:Journal Article
- Keywords:
@ Qingxin Sushitie/chemistry;
Indigo carmine/analysis;
Chromatography, high pressure liquid
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for the determination of indigo content in Qingxin Sushitie by HPLC. Methods At 30℃, HPLC was performed to determine indigo on ODS column(4.6?250 mm), the mobile phase was composed of methanol-acetonitrile-0.1mol/L ammonium acetate(60: 4: 36), flow rate was 1.0 mL/min; injection volume was 20 ?L and detection wavelength at 280 nm. Results The linearity of indigo was good within 0.44～6.60 ?g/mL (r=0.9991), the average recovery was 103.6% and RSD=1.11%. Conclusion This method is simple, sensitive and accurate, and can be used for quality control of Qingxin Sushitie.
