Determination of 2,3,5,4′-tetrahydroxystilbene-2-O-?-D-glucoside in Polygonum multiflorum Thumb. and Jiangzhitongmai Tablet by HPLC
- VernacularTitle:HPLC法测定制首乌及降脂通脉片中2,3,5,4′-四羟基二苯乙烯-2-O-?-D葡萄糖苷的含量
- Author:
Linmei PAN
;
Xiaoya XU
- Publication Type:Journal Article
- Keywords:
2,3,5,4′-tetrahydroxystilbene-2-O-?-D-glucoside;
Jiangzhitongmai Tablet;
HPLC
- From:
Chinese Traditional Patent Medicine
1992;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish the assay method of 2,3,5,4′-tetrahydroxystilbene-2-O-?-D-glucoside in Jiangzhitongmai Tablet (Radix Polygoni Multiflori, Rhizoma Alismatis, etc.). Methods:HPLC conditions were as fellows: Chromasil C 18 column, a mobile phase of CH 3CN-H 2O(25∶75), and the wavelength at 320nm. Results:2,3,5,4′-tetrahydroxystilbene-2-O-?-D-glucoside was linear within the range of 0.044～ 0.7 ?g with a correlation coefficient of 0.9999. The average recovery was 98.54%. Conclusion:The method is simple, swift, accurate and practical in quality control of Jiangzhitongmai Tablet.
