Determination of phellodenrine in Cortex Phellodendri by HPLC
- VernacularTitle:HPLC法测定黄柏中黄柏碱的含量
- Author:
Deqing ZHOU
;
Zhixiong GUO
;
Zeyuan LUO
;
Aijun ZHANG
;
Zhifan HUANG
- Publication Type:Journal Article
- Keywords:
Cortex Phellodendri;
phellodendrine;
HPLC;
assey
- From:
Chinese Traditional Patent Medicine
1992;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To determine phellodendrine in Cortex Phellodendri by HPLC. Methods : HPLC condition consists of C 18 column (Phenomenex, 150mm, 5?), actonitrile:250mL of 0.05mol?L -1 phosphoric acid +0.4mL of diethylamine (9∶91, v/v) as mobile phase, detection wavelength at 284nm. Results : The averagy recovery of phellofendrine was 101.43% ( RSD =1.56%, n =5) and the linear range of phellodrine was 0.4368～2.1840?g??L -1 , r =0.9998. Conclusion : The method is simple, accurate and reproducible, and can be used for the determination of phellodendrine in Cordex Phellodendri.
