The protective effect of exercise on osteopenia in ovariectomized rats
- VernacularTitle:运动对去势雌性大鼠骨质疏松的保护作用
- Author:
Jing LI
;
Yibin CHEN
;
Ying ZHANG
- Publication Type:Journal Article
- Keywords:
Exercise;
Osteoporosis;
Apoptosis;
IGF-1;
Osteocyte
- From:
Chinese Journal of Physical Medicine and Rehabilitation
2003;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effect of exercise on osteopenia female rats caused by ovariectomy and its mechanism. Methods Twenty-four female rats aged 6 months were randomly divided into 3 groups: control group(CON),ovariectomized model group(OVX) and exercise group(Ex).The ovariectomy operation was performed with rats in OVX and Ex groups. Rats in Ex group were trained on a treadmill at a velocity of 18m/min for 1h, once daily for 5 days a week. Each group of rats were sacrificed at 12 weeks postoperatively and Dual-energy X-ray absorptiometry was carried out to measure the bone mineral density(BMD) of the femur. IGF-1 proteins in trabecular bone of proximal femur were examined by using immunochemical stain and the intensity of IGF-1 was determined by use of imaging analysis techniques. The apoptotic osteocytes in the trabecular bone of proximal femur were detected by using terminal-deoxynucleotidyl mediated nick end labeling (TUNEL) technique. Results The BMD of CON, Ex and OVX group was (0.170?0.011)g/cm2,(0.154?0.013)g/cm2 and(0.167?0.013)g/cm2, respectively. The difference of the BMD level between Ex and CON groups was not significant, however, the BMD level in OVX group was significantly lower than that of the Ex and CON groups (P
