RESPONSE AND RELATIONSHIP OF THE SPINAL CORD ASTROCYTES AND NEURONS TO PAIN INDUCED BY UNILATERAL TIBIA AND FIBULA FRACTURE IN RAT
- VernacularTitle:大鼠腰髓背角星形胶质细胞及神经元对一侧胫、腓骨骨折的反应及其相互关系
- Author:
Hui ZHANG
;
Guangyun ZHANG
;
Li DUAN
;
Zhiren RAO
- Publication Type:Journal Article
- Keywords:
Neuron;
Astrocyte;
Spinal cord;
Fracture;
Immunohistochemistry;
Rat
- From:
Acta Anatomica Sinica
2002;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the instant reactions and relationship of astrocytes(AS) and neurons in rat spinal cord after the unilateral tibia and fibula bone fracture. Methods With single or double immunohistochemical staining method,the expression of Fos-like immunoreaction(-LI), glial fibrillary acidic protein(GFAP-LI), and protein kinase C(PKC-LI) were observed. Results Here we showed 1^ After the nociception of the treatment the PKC-LI,Fos-LI astrocytes and neurons,and GFAP-LI astrocytes were distributed in dorsal horn of rat lumbar spinal cord,predominantly in the superficial layer.2^ The distribution of Fos-LI neurons and Fos/GFAP-LI AS in dorsal horn of rat lumbar spinal cord were similar,there were close relationship between Fos-LI neurons and GFAP/Fos-LI AS.3^ The peak time of GFAP/Fos-LI AS expressive was 45?min after fracture,while that of Fos-LI neurons was 90?min,the peak time of PKC-LI astrocytes expressive was earlier than that of PKC-LI neurons.Conclusion The AS as well as neurons in dorsal horn of rat lumbar spinal cord might be involved in the instant response and adjustment of the lower extremity bone fracture nociception simultanneously.
