THE MECHANISMS OF IBMX AND CPT-cAMP ON THE REGENERATION OF RGCs
- VernacularTitle:3-异丁基-1-甲基黄嘌呤和CPT-环磷腺苷促进视网膜节细胞再生的机制探讨
- Author:
Yonghong ZHU
;
Haibiao LI
- Publication Type:Journal Article
- Keywords:
CPT-cAMP;
IBMX;
Retinal ganglion cell;
Regeneration;
Golden hamsher
- From:
Acta Anatomica Sinica
2002;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective Use three inhibitors to block three different signaling pathway to explore the mechanisms of 3-isobutyl-1-methylxanthine(IBMX) and 8 (4 Chlorophenylthio) cAMP(CPT-cAMP) on the regeneration of retinal ganglion cells. Method Fluorescent retrogratde tracing method and quantitative anatomical techniques were used to measure the numbers of RGCs in control one,control two group and experiment groups. Results 1^ In control group 1(AG),the mean number of regenerating RGC was 1428??284/retina in 4 weeks after surgery; 2^ In control group 2(AG+IBMX+CPT-cAMP) the mean number of regenerating RGC was 4?917?1 489/retina in 4 weeks after surger; 3^ The mean number of regenerating RGC of experiment groups in 4 weeks after surgery were (1) H89 group(AG+IBMX+cAMP+H89):1?426?320/retina; (2)Wortmannin group(AG+IBMX+cAMP+Wortmannin):4143?1?057/retina; (3)PD98059 group(AG+IBMX+cAMP+PD98059):4?154?965/retina. Conclusion The promoting effects of IBMX and CPT-cAMP on regeneration of RGCs can be interrupted by H89 and can't be blocked by wortmannin and PD98059.
