Experimental study on embryo culturing medium of malignant tumor growth fluid
- VernacularTitle:恶性肿瘤细胞生长液培养早期胚胎的实验研究
- Author:
Zhu YANG
- Publication Type:Journal Article
- Keywords:
GRC-1;
Culture;
Mouse;
Embryo
- From:
Journal of Chongqing Medical University
2003;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To find a good culture medium for embryo development in-vitro.Methods:D4 mouse embryo was cultured with carcinoma of kidney GRC-1 growth fluid and FD+20% fetal calf serum respectively in-vitro and the rate of zona-hatching,attachment and trophoblast outgrowth were compared respectively.Results:The rate of zona-hatching,attachment and trophoblast outgrowth with GRC-1 were 89.68%,86.51 %和84.92%,respectively.These were significantly higher than those of the control group ( P
