The influence of MDR1 gene therapy on the survival rate of few late hepatocarcinoma balb/c mouse
- VernacularTitle:多药耐药(MDR1)基因治疗对中晚期肝癌小鼠生存率的影响
- Author:
Chunbao GUO
- Publication Type:Journal Article
- Keywords:
Gene therapy;
Multidrug resistance;
Doxorubicin;
Hepatocarcinoma;
Marrow transplant
- From:
Journal of Chongqing Medical University
2003;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:The study was to improve the protection of the bone marrow during the chemotherapy of late hepatocarcinoma balb/c mice and increase its survival rate. Methods:Cultivated H22 hepatocarcinoma cell was injected into the balb/c mouse to make the animal model. MDR1 gene was transferred into the hematopoietic cells of murine bone marrow by the mediation of retrovirus vecter and transplanted into the balb/c mouse with hepatocarcinoma exposure with 60 Co-? ray in advance.WBC were investigated 3 days after high-dose chemotherapy. The function and the expression of MDR1 gene were detected by PCR method and IC method in vitro and in vivo. Results:WBC of the different dose experiment group was higher than that of the control group obviously ( P 0.05) ,but in the second week WBC counting of the 0.8mg/kg group was lower than that of the other two groups( P
