A study on specific mRNA related to the heroin dependence
- VernacularTitle:与海洛因依赖有关的特异性mRNA研究
- Author:
Huaqing MENG
- Publication Type:Journal Article
- Keywords:
Heroin dependence;
mRNA;
Suppressive subtractive hybridization
- From:
Journal of Chongqing Medical University
2003;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To find out the specific gene related to heroin dependence.Methods:Heroin-dependent models in mice were established by dose-increasing administration.After withdrawal syndrome was precipitated by naloxone,total RNA and mRNA were isolated from the brains of experimental and control groups respectively,then suppressive subtractive hybridization was proceeded.The hybridization sample was amplified by PCR and analyzed on a agarose/EB gel.Results:The mRNA level of the experimental group was significantly higher than that of control group and a differently expressing cDNA fragment was obtained from the brain of the experimental group.Conclusion:There is probably an expression of the specific gene in the brain of heroin-dependent mice.
