Effect of Astragalus Polysaccharide in Stimulating the Secretion of Hematopoietic Growth Factors from Activated Human PBMC
- VernacularTitle:黄芪多糖对有核细胞分泌造血细胞因子的影响
- Author:
Xiaofen LOU
;
Binghua ZHANG
;
Jing SONG
;
Bin LIU
;
Xiaoling DENG
- Publication Type:Journal Article
- Keywords:
Astragalus polysaccharide/pharmacology;
Monocytes/drug effects;
Lymphocytes/drug effects;
Granulocyte colony-stimulating factor;
Granuloeyte-macrophge colony-stimulating factor;
Cell culture
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
2000;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of Astragalus Polysaccharide (for intravenous injection) in stimulating the secretion of hematopoietic growth factors from activated human peripheral blood mononuclear cells (PBMC). Methods Human PBMC were isolated from concentrated leukocytes and were cultured with Astragalus Polysaccharide. Hematopoietic growth factors such as granulocyte colony-stimulating factor (G-CSF) and granulocyte-macrophage colony-stimulating factor (GM-CSF) in the supernatant were determined by enzyme-linked immunosorbent assay (ELISA). Results Astragalus Polysaccharide stimulated G-CSF and GM-CSF formation in a dose-dependent and a dose-effect and time-dependent manner. Conclusion Astragalus Polysaccharide can stimulate the secretion of hematopoietic growth factors from activated human PBMC, indicating its clinical application for leukocytopoiesis.
