Fingerprint analysis of Cortex Phellodendri by RP-HPLC
- VernacularTitle:黄柏HPLC指纹图谱鉴别研究
- Author:
Chenchen ZHU
;
Jianxia MO
;
Chaozhan LIN
- Publication Type:Journal Article
- Keywords:
Cortex Phellodendri/chemistry;
@alkaloids of Cortex Phellodendri/analysis;
Chromatography, high pressure liquid;
@Fingerprint
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
2000;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a new method for the identification of Cortex Phellodendri. Methods A HPLC fingerprint at 220 nm has been established for identification of Cortex Phellodendri, using a Merck-Lichrospher RP-C_(18) column(4.6?250mm, 5?m) and ACN(A) with the buffer of 0.3% H_3PO_4-NH(CH_2CH_3)_2 (B) in gradient as the mobile phase. The results were compared with the chromatograms of three species of Rbizoma Coptidis under the same conditions. Results HPLC fingerprint of Cortex Phellodendri at 220nm consists of 14 specific peaks. The steady appearance of the peaks and their relative contents were considered as important signs for the identification of this crude drug. The chromatograms of Rhizoma Coptidis were obviously different from the fingerprint of Cortex Phellodendri. Conclusion This method has been proven to be feasible for the identification of Cortex Phellodendri.
