Determination of Schizandrin, Deoxyshizandrin and ?-Schizandrin in Jiangtang Soft Capsule by HPLC
- VernacularTitle:HPLC法测定降糖软胶囊中五味子醇甲、甲素和乙素的含量
- Author:
Shudan ZHANG
;
Sheng NI
;
Aizhi YUAN
- Publication Type:Journal Article
- Keywords:
@ Jiangtang soft capsule/chemistry;
@ Schizandrin/analysis;
@ Deoxyschizandrin/analysis;
@ ?-Schizandrin/analysis;
Chromatography, high pressure liquid
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
2000;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for the determination of schizandrin, deoxyschizandrin and ?-schizandrin in Jiangtang soft capsule. Methods The sample was extracted by methanol. The chromatographic conditions were: a gradient mobile phase of methanol-water(65: 35)within 0～20 rain and methanol-water(70: 30)within 20～80 min, the wavelength at 250 nm. Results A linear range of schizandrin, deoxyschizandrin and ?-schizandrin was within 0.71 ?g～3.53 ?g(r=0.99998, n=5), 0.19?g～0.95 ?g(r=0.99993, n=5)and 0.36?g～1.80?g(r=0.99997, n=5) and the average recoveries were 100.44%, 98.06% and 101.14% respectively. Conclusion This method is easy, sensitive, specific and accurate for the determination of schizandrin, deoxyschizandrin and ?-schizandrin in Jiangtang soft capsule.
