Apoptosis gene expression profiling of placental trophoblast cells in patients with pregnancy induced hypertension
- VernacularTitle:妊娠高血压综合征患者胎盘滋养细胞凋亡基因表达谱的研究
- Author:
Lizhou SUN
;
Wenying ZHAO
;
Lei HONG
;
Tao PENG
- Publication Type:Journal Article
- Keywords:
Pregnancy complications, cardiovascular;
Hypertensions;
Placenta;
Apoptosis;
Gene expression profiling
- From:
Chinese Journal of Obstetrics and Gynecology
2000;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the function of placental trophoblast cell apoptosis on the pathogenetic mechanism of pregnancy induced hypertension (PIH). Methods Apoptosis of trophoblast cells in 20 cases of PIH(PIH group) and in 10 cases of normal pregnancy (control group) were directly observed using the terminal-deoxynucleotidyl transferase mediated d-UTP nick end labeling (TUNEL) method. Apoptosis gene expression patterns were screened with gene chip provided by Poxing Company, Shanghai. Standards for differently expressed genes were: (1) An absolute value of the natural logarithm of cy5(PIH group)/cy3(control group) greater than 0.69 with a difference of signal of cy5 2 times over that of cy3. (2) The signal value either cy3 or cy5 must be greater than 800. Results (1) TUNEL test showed that the number of trophoblast cells apoptosis per ten thousand ?m 2 was 1.584 in the PIH group and 0.032 in the control group with significant difference between the two groups (P
