EFFECT OF NERVE REGENERATION FACTOR ONAPOPTOSIS OF PC12 CELLS
- VernacularTitle:神经再生素对PC12细胞凋亡的影响
- Author:
Gang CHEN
;
Xiaodong WANG
;
Peiyun ZHANG
;
Fei DING
- Publication Type:Journal Article
- Keywords:
NRF;
Apoptosis;
Annexin V;
caspase-3;
PC12 cells
- From:
Acta Anatomica Sinica
2002;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of nerve regeneration factor(NRF) on apoptosis of PC12 cells and its mechanism. Methods PC12 cells under the condition of low concentration serum(0^3% newborn bovine serum in RPMI 1640 medium) were treated with NRF or NGF(both are concentrations of 0^01*!mg/L,0^1*!mg/L,and 1*!mg/L).The changes in the number and outgrowth in cultured PC12 cells were observed under light microscope,and the surival and growth of cultured PC12 cells were measured by MTT.The percentage of apoptosis or necrosis cells and caspase-3 activity were both detected by FCM. Results There were no difference in these parameters between the group of NRF and NGF,whereas there were superior to that of the blank control group.Conclusion NRF can protect the cultured PC12 cells from apoptosis in low concentration serum.
