THE PROMOTIVE EFFECT OF SERTOLI CELL ON NEURONS in vitro
- VernacularTitle:睾丸支持细胞促进体外培养神经元生长的研究
- Author:
Fanfang SHANGGUAN
;
Xiaolin SHI
- Publication Type:Journal Article
- Keywords:
Sertoli cells;
Cell culture;
Neurons
- From:
Acta Anatomica Sinica
2002;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the promotive effect of SCs on neurons in vitro Methods SCs feeder layer was made as conditioned medium(SCM), then used in coculture with neurons respectively. Count the survived neurites of the cultured cell and observe the cell size by a medical image analyzer. Results The number of surviving cells, neurites outgrowth and cell size cocultured either with SCs feeder layer or SCM was markedly increased than that of control cultures(P
