THE DEVELOPMENT AND IDENTIFICATION OF OSTEOBLASTS FROM RAT BONE MARROW STROMAL CELLS CULTURED in vitro
- VernacularTitle:体外培养大鼠骨髓基质细胞分化为成骨细胞新方法的建立和鉴定
- Author:
Xi MAO
;
Zhao MAO
;
Jianju WANG
- Publication Type:Journal Article
- Keywords:
Bone marrow stromal cells;
Osteoblasts;
Culture in vitro;
Rat
- From:
Acta Anatomica Sinica
2002;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective The purpose of this study was to develop a new cultural method, so that the rat bone marrow stromal cells (MSCs) can well differentiate into osteoblasts in vitro and the cultured osteoblasts can be identified. Methods MSCs of rats were isolated and cultured, then the cultured cells were identified with inverted microscopy and undergone an HE stain for observing the morphology of living cells, and the cells were histochemically stained for examining the activity of cellular alkaline phosphatase (ALP), as well as were radio-immunologically analyzed for detecting the secretion of osteocalcin. Results The MSCs were typical fibroblast-like and possess a strang proliferative capability after cultured in vitro.The cellular ALP activity and osteocalcin secretion achieved the peak value from the fortieth day and maintained the status until the sixtieth day after cultured in vitro.Conclusion The data demonstrated that the new developed culture method has the advantages of short time, less risk of contamination and higher efficiency, and conducive to MSC's differentiating and proliferating into osteoblasts that do function well.
