Impact of dehydroepiandrosterone on antioxidant capacity of Raji cell cultured in vitro
- VernacularTitle:脱氢表雄酮对体外培养Burkit淋巴瘤细胞抗氧化能力影响的研究
- Author:
Detai ZHANG
- Publication Type:Journal Article
- Keywords:
Dehydroepiandrosterone;
Raji cell;
Glucose-6-phosphate dehyarogenase
- From:
Journal of Medical Postgraduates
2003;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the influence of dehydroepiandrosterone(DHEA) on antioxidant capacity of Raji cell cultured in vitro. Methods: Raji cell line was cultured in vitro in the presence of DHEA and/or phenazine methosulfate(PMS), some antioxidant enzymes including glucose-6-phosphate dehydrogenase activity(G-6-PD),glutathione peroxidase activity(GPX), glutathione reductase activity(GR) and intracellular glutathione(GSH) concentration of the DHEA pretreatment cells were evaluated. meanwhile, these results were compared with that from the respective control cells. Results:(G-6-PD) activities were suppressed significantly and no changes in activities of GPX,GR and intracellular GSH concentration were occurred while Raji cells were cultured in the presence of DHEA at concentration more than 5.0?mol/L . On the other hand, the DHEA pretreatment cells show no changes in (G-6-PD,)GPX,GR activities and significantly decreased GSH concentration(P
