In-Vitro Induction of Mesenchymal Stem Cells Differentiation intoNeurons in Adult Rats by Serum ContainingCarapax et Plastrum Testudinis
- VernacularTitle:龟板含药血清体外诱导成年大鼠骨髓间充质干细胞分化为神经元
- Author:
Dongfeng CHEN
;
Shaohui DU
;
Yhuei LI
;
Hui LI
- Publication Type:Journal Article
- Keywords:
CARAPAX ET PLASTRUM TESTUDINIS/phaimacology;
BONE MARROW CELLS/ ultrastructure;
STROMAL CELLS/ ultrastructure;
NEURONS/ultrastructure;
CELL DIVISION/drug effects
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2001;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
[Objective] To observe the effect of serum containing Carapax et Plastrum Testudinis (CPT) in inducing the differentiation of mesenchymal stem cells (MSC) of adult rats into neurons. [Methods] The fifth generation of MSC were induced by the serum containing CPT (sero-CPT) and the expressions of neurofilament (NF) protein and glial fibrillary acidic protein (GFAP), the marks of neuron-like cells were detected by immunohistochemical method. [Results] The expression of NF protein was positive after the stimulation of sero-CPT and the expression reached the peak 12 hours after induction. [Conclusion] Sero-CPT can induce the differentiation of MSC into neurons in vitro.
