Reversing drug resistance of human erythroleukemia cell line K562/A02 on DOX by total saponings Panax ginseng
- VernacularTitle:人参总皂甙逆转K562/A02细胞对DOX耐药性的研究
- Author:
Li WANG
- Publication Type:Journal Article
- Keywords:
Total saponins Panax ginseng;
Cell line,K562;
Drug resistance;
mdr-1:bcl-2
- From:
Journal of Chongqing Medical University
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To research the effect of total saponins Panax ginseng (TSPG) on the drug resistance of human erythroleukemia drug resistant cell line K562/A02 and its mechanism.Methods:The sensitivity of TSPG-treated K562/A02 cells to anticancer drugs was determined by MTT assay.Immunohistochemistry method was used to measure mdr-1 gene product P-gp and apoptosis regulation gene product bcl-2 expression. RT-PCR technique was used to examine mdr-1 mRNA expression.Results:In non-toxic dosages, TSPG strengthened the toxicity effect of doxorubicin on K562/A02 cells, while the reversing effect of TSPG was related to its concentration. Decrease in P-gp expression in K562/A02 cells was marked ( P O. 05)after 3 days of TSPG(150?g/ml)treatment.Decrease in mdr-1 mRNA expression was significant ( P
