Leptin-mRNA expression in placental tissues:relationship with fetal growth and development
- VernacularTitle:胎盘瘦素mRNA表达水平及其与胎儿生长发育的相关性研究
- Author:
Hongbo QI
- Publication Type:Journal Article
- Keywords:
Leptin;
Placenta;
Fetus;
Reverse transcription polymerase chain reaction
- From:
Journal of Chongqing Medical University
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate whether placental tissues produce leptin and determine the relationship between expression levels of leptin-mRNA in placenta and fetal growth and development.Methods:52 neonates were divided into 3 subgroups:(1)birth weight large for gestational age (LGA)( n =14),(2)birth weight appropriate for gestational age (AGA)( n =21),(3)birth weight small for gestational age (SGA)( n =17).Reverse transcription-polymerase chain reaction(RT-PCR) technique was used to detect human leptin-mRNA derived from placental-RNA.The expression levels of leptin-mRNA were quantitated using fluorescence intensity analysis.The correlations of expression level of placental leptin-mRNA with placental weight,birth weight,newborn Ponderal index(PI),maternal weight,maternal body mass index(BMI) and gender were studied.Results:The expression levels of placental leptin-mRNA in LGA infants(119.7?13.0) and SGA infants(76.8?15.1) were significantly higher and lower respectively than those in the AGA infants(94.4?17.4)( P
