Methodology of separation, purification, cultivation and identification of rat bone marrow macrophage
- VernacularTitle:大鼠骨髓巨噬细胞的分离,纯化,培养以及鉴定
- Author:
Jing LI
- Publication Type:Journal Article
- Keywords:
Bone marrow macrophage;
Separation;
Purification;
Identification
- From:
Journal of Chongqing Medical University
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the biological functions of bone marrow macrophage (BMM ?) and to establish the methodology of separation, purification, cultivation and identification of BMM ?.Methods:Using the techniques of anchorage-dependent culture of separated rat bone marrow cell (rBMC) in DMEM culture media (contain 20% horse serum,20% (v/v)L 929 conditioned media) in vitro,a lot of purified anchor cells were obtained, and these cells were identified with specifically biological marker of macrophage, such as 1, morphologicalobservation:invert phase contrast microscopy, light and electron microscopy;2, enzyme cytochemistry:acid phosphatase (ACP),?-acetic acid naphthol esterase (?-ANE);3,phagocytic experiment:phagocytosis of chicken erythrocytes and prepared Chinese ink;4,immunocytochemistry: surface specific antigen of macrophage ( CD 68 stain).Results:The cells were purified having functional satisfactory macrophage according to morphological observation,enzyme cytochemistry, phagocytic experiment and immunocytochemistry.Conclusion:This is a simple and easy method for separation, purification, cultivation and identification of rat marrow macrophage.
