Expression of Toll-like receptor 4 in kupffer cells in experimental alcohol-induced liver disease
- VernacularTitle:实验性酒精性肝病时Kupffer细胞Toll样受体4的表达
- Author:
Guoqing ZUO
- Publication Type:Journal Article
- Keywords:
Toll-like receptor;
Alcohol-induced liver disease;
Kupffer cells
- From:
Journal of Chongqing Medical University
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the synthesis of Toll-like receptor (TLR)4 protein and its mRNA expression in Kupffer cells (KCs) and evaluate the role of TLR 4 in the liver injury in the rats with alcohol-induced liver disease (ALD).Methods:Twenty eight Wistar rats were divided into two groups: ethanol-fed group(group E) and control group (group C). Group E were fed with ethanol (dose of 5～12g/kg/d) and group C received dextrose instead of ethanol. Rats of the two groups were sacrificed at 4 wk and 8 wk. KCs were isolated and incubated.TLR 4 protein in KCs was determined by laser scanning confocal microscopy (LSCM).TLR 4 mRNA in KCs was determined by the reverse transcription polymerase chain reaction (RT-PCR).Plasma endotoxin levels were measured using the Limulus Amebocyte Lysate test kit.The liver pathology was observed under light and electronic microscopy.Results:The fluorescence intensity of TLR 4 protein in KCs by LSCM was stronger in group E than those in group C. Ethanol administration led to a significant increase in TLR 4 mRNA expression compared with group C ( P
