Comparison of Anti-mycobacterial Drug Susceptibility Test Results by Institutes and Methods.
10.5145/KJCM.2008.11.1.43
- Author:
Seung Hwan OH
1
;
Young Jin KIM
;
Seung Kyu PARK
;
Sang Hyun HWANG
;
Hyung Hoi KIM
;
Eun Yup LEE
;
Chulhun L CHANG
Author Information
1. Department of Laboratory Medicine, Pusan National University School of Medicine, Busan, Korea. CCHL@pusan.ac.kr
- Publication Type:Original Article
- Keywords:
Mycobacterium tuberculosis;
Drug susceptibility tests;
Culture Media
- MeSH:
Academies and Institutes;
Agar;
Aminosalicylic Acid;
Culture Media;
Cycloserine;
Humans;
Isoniazid;
Mycobacterium tuberculosis;
Ofloxacin;
Prothionamide;
Quality Control;
Rifampin;
Sensitivity and Specificity;
Streptomycin
- From:Korean Journal of Clinical Microbiology
2008;11(1):43-48
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: The purposes of the current study were to evaluate the concordant rates of anti-mycobacterial drug susceptibility test (DST) results in different solid media performed in different institutes, and to determine reliable susceptible testing methods. METHODS: One hundred and twenty two Mycobacterium tuberculosis strains were isolated from patients in A Hospital in 2005. DSTs were performed by the absolute concentration method using L?wenstein Jensen medium in both A Hospital (method A-1) and B Institute (method B-1) and by the proportion method using Middlebrook 7H10 agar in B Institute (method B-2). Nine drugs were used including isoniazid and rifampin. Sensitivity and specificity of each method were estimated by using the acceptable standard of 90% for isoniazid and rifampin and 80% for other drugs. The therapeutic outcomes of quinolone-administered patients were evaluated according to ofloxacin susceptibility results. RESULTS: Method B-1 showed sensitivity and specificity levels over the acceptable standard levels for all drugs. Method B-2 showed specificity lower than the acceptable levels for rifampin and cycloserine. Method A-1 showed specificity lower than the acceptable levels for isoniazid, streptomycin, p-aminosalicylic acid, and ofloxacin and sensitivity lower than the acceptable levels for prothionamide and cycloserine. The concordance rates of therapeutic outcomes with method B-1, method B-2, and method A-1 were 77%, 74%, and 65%, respectively. CONCLUSION: The drug susceptibility results for some drugs were discordant between the testing laboratories and media, requiring an urgent application of quality control programs to raise the reliability of anti-mycobacterial DST.
