Growth-inhibiting effects of Taxol on QGY cells:G_2/M arrests and apoptosis
- VernacularTitle:紫杉醇对QGY细胞的作用:G_2/M期阻滞和细胞凋亡
- Author:
Song HE
- Publication Type:Journal Article
- Keywords:
Taxol;
Hepatoma;
Apoptosis
- From:
Journal of Chongqing Medical University
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of Taxol on QGY human hepatoma and its mechanism.Methods:The hepatoma cell growth was measured by MTT-microculture tetrazolium assay.Cell-cycle kinetics and apoptosis were analyzed by flow cytometry and microscopic examination.Results:Taxol inhibited the hepatoma cell growth in concentration- and time-dependent manners.Marked cells accumulated in G 2/M phase. The rate of cell growth inhibition was 78.6% after intervention of 56?mol/L Taxol for 72 hours. The cells showed G 2/M arrest and apoptosis.Conclusion:Taxol suppresses the growth of QGY human hepatoma cells in vitro by causing cell-cycle arrest, and apoptosis of the human hepatoma cells. The mechanisms of Taxol provide theoretical guidance for clinical treatments.
