HPLC determination of adenosine phosphates in mouse myocardium
- VernacularTitle:高效液相色谱法测定心肌腺苷酸含量
- Author:
Jianbin CHEN
- Publication Type:Journal Article
- Keywords:
High performance liquid chromatography(HPLC);
Adenosine phosphate
- From:
Journal of Chongqing Medical University
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:A method for the quantitative determination of adenosine phosphates of mouse myocardium by HPLC is described.Methods:A ODS C 18 column and a mobile phase of methol-50mmol?L -1 phosphate (1∶99)buffer solution (pH 6.07),with UV detector at 254nm were used.Results:The average recovery rates of ATP,ADP and AMP were 101.7%?9.3%,94.8%?10.6% and 98.3%?5.7% respectively;the RSDs of within-day were 3.5%,6.8% and 4.8%,and the RSDs of between-days were 6.4%,8.6% and 8.2%,respectively.Conclusion:The method is simple,rapid and senstive and can be used to analyse the adenosine phosphates in myocardium.The myocardial contents of ATP,ADP as well as AMP decline notably in mice with viral myocarditis.
