Determination of 5,7-dimethoxycoumarin in Fructus Citris Sarcodactylis by HPLC
- VernacularTitle:HPLC法测定广佛手中5,7-二甲氧基香豆素的含量
- Author:
Youheng GAO
;
Yuanming DIAO
;
Xinsheng PENG
;
Honghua XU
- Publication Type:Journal Article
- Keywords:
@ Fructus Citris sarcodactylis/chemistry;
@5,7-dimethoxycoumarin /analysis;
Chromatography,high pressure liquid
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
1993;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the content of 5,7-dimethoxycoumarin in Fructus Citris Sarcodactylis. Methods HPLC was used. Mobile phase was methanol-water(65 ∶35),detection wavelength at 326 nm,flow rate 1mL/min and column temperature at 35 ℃.Results The linearity of 5,7-dimethoxycoumarin was in the range of 0.3528～1.7640 ?g. The regression equation was Y=2403.73 X-65.35,r=0.9998. The average recovery was 99.33 %,RSD=1.47 %(n=6). Conclusion The method is simple and accurate. It can be applied in the determination of 5,7-dimethoxycoumarin in Fructus Citris sarcodactylis.
