Determination of Naringin in Gongning Granule by HPLC
- VernacularTitle:HPLC法测定宫宁颗粒中柚皮苷的含量
- Author:
Ying FENG
;
Sheng NI
;
Qiang XU
- Publication Type:Journal Article
- Keywords:
Gongning granule/chemistry;
@Naringin/analysis;
Chromatography,high pressure liquid
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
1993;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for the determination of naringin in Gongning Granule, Methods The sample was extracted with methyl and the chromatographic conditions were as follows: Kromasil C18 chromatographic column(250 mm?4.6 mm ,5 ?m)with a mobile phase of methyl and 1 %acetic acid (35.5 ∶64.5),the detection wavelength at 283 nm and the flow rate being 1.0mL?min-1. Results A linearity of naringin in Gongning Granule was obtained in the range of 0.0980 ?g to 0.980 ?g,r=0.9997 (n=6).The average recovery was 98.7 %and RSD=1.96 %(n=6). Conclusion This method is easy,sensitive and accurate for the determination of naringin in Gongning Granule.
