Determination of tetrahydropalmatine in Juyanbaokang Granules by SPE-HPLC
- VernacularTitle:SPE-HPLC法测定菊延保康颗粒中延胡索乙素的含量
- Author:
Lei CHEN
;
Yongqing LI
;
Jihong ZHU
;
Wenyin LIU
- Publication Type:Journal Article
- Keywords:
Juyanbaokang Granules;
tetrahydropalmatine;
solid phase extraction (SPE);
HPLC
- From:
Chinese Traditional Patent Medicine
1992;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To determine tetrahydropalmatine in Juyanbaokang Granules (Rhizoma Corydalis, Radix Aconcti Lateralis Preparata, Radix Rauwolfiae, etc.) by SPE-HPLC. Methods : Samples were purified by C 18 SPE column. HPLC column was Dikma Diamonsil C 18 (250mm?4.6mm,5?m), mobile phase was 0.05M potassium dihydrogen phosphate-0.005M sodium heptanesulfonate-acetonitrile (1.2∶1.2∶1), flow rate was 1.2 mL?min -1 and detection wavelength was 230nm. Results : The calibration curve was linear in the range of 0.067 ～0.402?g for tetrahydropalmatine ( r =0.9997). The average recovery was 98.66%. Conclusion : The method with good separation is convenient, rapid, accurate and reliable.
