Detection of HCV RNA in Plasma/Serum from Donors by NASBA
- VernacularTitle:应用NASBA技术对献血者血浆/血清进行HCVRNA检测
- Author:
Ruxin HUANG
;
Chunhong ZHAO
;
Changrong CHEN
- Publication Type:Journal Article
- Keywords:
NASBA;
HCV RNA;
Hepatitis C,posttransfusion
- From:
Chinese Journal of Blood Transfusion
1988;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyse the necessity and feasibility of HCV RNA detection for donor screening.Methods Plasma from 50 donors was mixed into a minipool, the nucleic acid was extracted by NucliSens Extractor and amplified by NASBA, the amplification products were detected by ECL. A performance control and a positive control were coupled with the samples through storage, nucleic acid extraction, amplification and detection.Results Two specimens were found HCV RNA positive in the 10000 anti HCV negative samples.Conclusion HCV RNA screening of plasma minipools by NASBA may prevent hepatitis C virus infection.
