Extraction of Prekallikrein Activator
- VernacularTitle:激肽释放酶原激活剂的提取
- Author:
Yaqin CHENG
- Publication Type:Journal Article
- From:
Chinese Journal of Blood Transfusion
1988;0(01):-
- CountryChina
- Language:Chinese
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Abstract:
The FactorⅫ(FXII or a-Ⅻa)in Cohn's FIV was adsorbed by diato- mite,and then the FXⅡ(or ?-Ⅻa) was activated into ?-Ⅻa(Hageman factor fragment,PKA,MW31000)by the co-adsorbed kallikrein with sub- sequent releasing it toabuffer solu- tion.Finally,the product was purified by DEAE-Sephadex A_(50).The activity of prepared PKA was 8 963IU/ml,and the preparation can lead to signifi- cant reduction in blood pressure in rats.
