The Establishment of a Human Myeloma Cell Line 8310——IBTCHM 8310 and Observations of Its Biological Properties
- VernacularTitle:人骨髓瘤IBTCHM8310细胞系的建立及其生物学特性的研究
- Author:
Heng CUN
- Publication Type:Journal Article
- From:
Chinese Journal of Blood Transfusion
1988;0(01):-
- CountryChina
- Language:Chinese
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Abstract:
A human myeloma cell line(IBTCHM8310) obtained from the bone marrow ofa patient who was diagnosed as multiplemyeloma has been repropagated to datefor 270 generations within 50 months.The cells recovered from cryopreserva-tion in liquid nitrogen for 17 times,grewand reproduced well.The growth curve shows that themultiplication of the cells were slow onthe first two days,then went straightly upand culminated on the 6th and 7th days.Chromosome analysis was performed on96 cells on metaphases of 164th generationand it was between 63-69.There was stilla prominent mode 65,66.Of each 1?10~5/mlcells suspension,0.5ml was injected in tra-peritoneally to nude mice,Significant tu-mor masses appeared 3 weeks after injec-tion.Pathological section showed that thecells were identical to those of their pa-rent line.
