EXPRESSION OF THE Nogo PROTEIN IN THE PRIMARY CULTURED ENSHEATHING CELLS FROM OLFACTORY BULB OF ADULT RAT BY CONFOCAL LASER SCANNING MICROSCOPE
- VernacularTitle:原代培养大鼠嗅球成鞘细胞Nogo(N-18)的共聚焦激光扫描显微镜观察
- Author:
Jun WANG
;
Ke WANG
;
Changman ZHOU
;
Enhua YU
- Publication Type:Journal Article
- Keywords:
Olfactory ensheathing cells;
Nogo;
Cell culture;
Confocal laser scanning microscopy;
Rat
- From:
Acta Anatomica Sinica
1957;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective Studying the expression of the Nogo(N\|18) in olfactory ensheathing cells(OECs) to investigate the functional relationship between Nogo and the OECs in promoting the regeneration of neurite axon. Methods The expression of the Nogo(N\|18) in the primary cultured ensheathing cells from olfactory bulb of the adult rats was studied with the method of immunocytochemical staining and double\|immunofluorescence staining examed under the confocal laser scanning microscope. Results The Nogo\|A protein was located in primary cultured ensheathing cells from the olfactory bulb.The protein was mainly distributed in the cytoplasm.The member and processes were less stained.Conclusion\ The ensheathing cells in vitro contain Nogo protein.It suggests that Nogo protein is not a depressive factor in the axon regeneration of olfactory system.\;[
