THE INDUCING EFFECTS OF GINKGOLIDE B ON NEURAL STEM CELLS DIFFERENTIATING INTO NEURONS
- VernacularTitle:银杏内酯B对成年大鼠神经干细胞向神经元分化的促进作用
- Author:
Zhen HUANG
;
Guohua JIN
;
Xinhua ZHANG
;
Meiling TIAN
;
Jianbing QIN
;
Huijun XU
- Publication Type:Journal Article
- Keywords:
Ginkgolide;
Neural stem cells;
Neuron;
Differentiation;
Adult rat
- From:
Acta Anatomica Sinica
1957;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effects of ginkgolide B on inducing neural stem cells differentiating into neurons. Methods A great deal of single cell clone neurospheres raised from single cell and proliferated by the technology of serum\|free culture and single cell clone.Suspensions of cell clone neurospheres were plated equably into 24 well plates and added into the 10% FBS differentiation medium containing ginkgolide B,BDNF or without any factor.Cultures were terminated at 7 and 14 days respectively.MAP\|2,neuron\|specific marker,were used to mark neurons by immunofluorescence.MAP\|2 positive neurons were observed and counted by fluorescence microscope.The area and perimeter of these positive neurons were analyzed. Results The number of MAP\|2 positive neurons in ginkgolide B group is more than that in the control group in two periods.The area and perimeter of MAP\|2 positive neurons in ginkgolide B group were markedly larger than those in the control group at 7 and 14 days after cultured,but it's less than those in the BDNF group.Conclusion\ Neural stem cells can be induced to differentiate into neurons by ginkgolide B which has the similar role to BDNF.\;[
