ACTIVATION OF caspase-3 DURING APOPTOSIS OF RAT CORTICAL NEURONS INDUCED BY ?-AP_(1-40)
- VernacularTitle:?淀粉样肽_(1-40)通过激活caspase-3诱导大鼠皮层神经元凋亡
- Author:
Limin CHEN
;
Xiaochun CHEN
;
Yuangui ZHU
;
Yican ZHOU
- Publication Type:Journal Article
- Keywords:
Beta\|amyloid peptide 1 40;
caspase\|3;
Cortical neuron;
Apoptosis;
Rat
- From:
Acta Anatomica Sinica
1957;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of caspase\|3 on ? ?? 1 40 induced apoptosis in rat cortical neurons. Methods Apoptosis was induced by 40?mg\5L -1 ?\|AP 1 40 .The apoptotic neurons were observed by TUNEL staining and DNA gel electrophoresis.The activity and mRNA expression of caspase\|3 was measured by fluorescent spectrofluorometer and RT\|PCR.The cleaved caspase\|3 was detected with immunocytochemistry. Results After treatment with ?\|AP 1\|40 ,the rat cortical neurons showed DNA fragmentation.The expression of caspase\|3 mRNA ratio to ? actin was 0\^78,0\^85 and 0\^39 respectively after treatment for 12,24 and 48?h.Caspase\|3 activity was 133 24?7 47,192 16?11 03,88 87?4 24 MFI\5?g protein -1 \5h -1 .The cleaved caspase\|3 was observed within cytoplasm.Specific inhibitor of caspase\|3 Ac\|DEVD\|CHO inhibited caspase\|3 activation and blocked cortical neurons apoptosis markedly.Conclusion\ Caspase\|3 might be an executor during ? AP 1 40 induced apoptosis in rat cortical neurons.[
