Identification of Rhizoma Alismatis by HPLC Combined with Evaporation Light Scattering Determination Method
- VernacularTitle:HPLC-ELSD法鉴别泽泻药材饮片
- Author:
Ying ZHANG
- Publication Type:Journal Article
- Keywords:
Rhizoma Alismatis/chemistry;
@Alisol A24-acetate/analysis;
@Alisol B23-acetate/analysis;
Chromatography,high pressure liquid;
@Evaporation light scattering detector
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
1993;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for the identification of Rhizoma Alismatis. Methods HPLC combined with evaporation light scattering detector(ELSD) was applied. Chromatographic column was Macherey-Nagel C18(250 mm?4 mm,10 ?m),acetonitrile -water (4 ∶1) used as mobile phase,flow rate 0.5 mL/min,gas flow rate of ELSD 2.25 L/min and the temperature of drift tube at 70 ℃. Results In the extraction of the authentic Rhizoma Alismatis,both alisol A24-acetate and alisol B23-acetate could be estimated;in the extraction of Rhizoma Alismatis from the market,the alisol A24-acetate could be estimated only;and only alisol B23-acetate could be estimated in the extraction of the standard powder of Rhizoma Alismatis. Conclusion The method is efficient,practical and easy to operate for detecting and identifying Rhizoma Alismatis.
