Determination of aesculin and aesculetin in periderm and leaves of Cortex Fraxini among different provenances
- VernacularTitle:不同地理种源秦皮的树皮及叶中秦皮甲素、秦皮乙素的含量测定
- Author:
Yueming ZUO
;
Zhenyue WANG
;
Honghua CUI
;
Limei LIU
- Publication Type:Journal Article
- Keywords:
HPLC;
Cortex Fraxini;
provenance;
aesculin;
aesculetin
- From:
Chinese Traditional Patent Medicine
1992;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To determine the contents of aesculin and aesculetin in periderms and leaves of Cortex Fraxini among differnet provenances. Methods: HPLC was used with acetonitrile-water(15∶85) as the mobile phase and detected wavelength at 348nm. C 18 column was adopted. Results: The calibration curves were linear. The value of correlation coefficient were 0.9992 and 0.9994, respectively. The average recovery of aesculin was 98.2% and aesculetin was 99.2%. RSD were 2.24% and 2.15%, respectively. The quality differences of Cortex Fraxini among different provenances were remarked. The quality of Cortex Fraxini from Shanxi province was the best. Conclusion: The method is applied in determination and analytics of content of Cortex Fraxini and is rapid, simple and easy to carry out. The method is with the feature of accuracy, repetition and stability.
