Quality study on evodiamine of Fructus Evodia by HPCE-FPS
- VernacularTitle:HPCE-FPS技术对吴茱萸质量的研究
- Author:
Qinyun LI
;
Zhihua WANG
;
Xiaokun HONG
- Publication Type:Journal Article
- Keywords:
Fructus evodia;
evodiamine;
proteins;
HPCE-CZE;
HPCE-FPS
- From:
Chinese Traditional Patent Medicine
1992;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: A HPCE capillary zone eletrophoretic method for the determination of evodiamine in Fructus Evodia was established. Similarly, areliable HPCE-FPS (fingerprint spectrum) was applied to compare quality of Fructus Evodia to 14 samples of 3 species from different areas. Methods: The evodiamine was detected on 254nm and proteins was on 196nm、254nm by HPCE. The electrophoretic parameters were optimized by studying the efffect of the buffer composition. Results: Calibration graph was constructed in the range 1.944 ?g?mL -1～7.766 ?g?mL -1 for evodiamine (r=0.9974). The regression equation was y= 188.7x-32.1. The average recovery was 101.3 (n=3). In this paper, the proteins of Frutus Evodia HPCE-FPS was established at 196nm and 254nm. Conclusion: Content of evodiamine from Fructus Evodia was determined by HPCE-CZE and HPCE-FPS and distinguished three species from different growing areas related to intrinsic quality of them. The research provides new technique for evaluating quality of traditional Chinese medicine.
