Effect of ginsenoside-Rb_1 and Re against cardiomyocyte apoptosis and expression of the related gene proteins in the experimental cardiac ischemia-reperfusion in rats
- VernacularTitle:人参皂甙Rb_1与Re抗大鼠实验性缺血再灌注心肌细胞凋亡及相关基因蛋白表达
- Author:
Hesong ZENG
;
Zhengxiang LIU
;
Xiaochun LIU
- Publication Type:Journal Article
- Keywords:
Ginsenoside Rb 1;
Ginsenoside Re;
Ischemia/reperfusion;
Cardiomyocyte apoptosis;
Gene expression
- From:
Chinese Journal of Physical Medicine and Rehabilitation
2003;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of ginsenoside Rb 1 and Re on cardiomyocyte apoptosis and expression of Bcl-2, Bax, Bad and Fas gene proteins after acute ischemia and reperfusion in rats and elucidate the possible mechanisms. Methods The ischemia-reperfusion heart model was made by ligating the left anterior descending branch of coronary artery in Wistar rats. The apoptotic cardiomyocytes were confirmed with transmission electron microscopy and counted with in situ nick labeling (TUNEL) method and light microscopy. The expression of Bcl-2?Bax?Bad and Fas gene proteins were studied by immunohistochemical staining. Mean optical density (OD) value of the gene proteins expression were quantitatively examined by image analysis system. Results A.The apoptotic cardiomyocytes were not observed in the sham-operation group. The number of the apoptotic cells were 134.45?45.61/field in the ischemia- reperfusion group, 51.65?13.71/field in the ginsenoside Rb 1-treated group and 90.66? 19.22/field in the ginsenoside Re-treated group, respectively. The differences were significant among the three groups (P0.05), but Bax?Bad and Fas gene expression were decreased significantly in the ginsenoside Rb 1 and Re-treated group as compared with the ischemia-reperfusion group (P
