Amplification of human diversified immunoglobulin genes from peripheral blood mononuclear cells
- VernacularTitle:从外周血单个核细胞扩增多样性人免疫球蛋白基因的研究
- Author:
Gang WANG
;
Yufeng LIU
- Publication Type:Journal Article
- Keywords:
Genes,immunoglobulin;
Monocytes
- From:
Chinese Journal of Rheumatology
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a usable method for the amplification of human diversified immunoglobulin genes.Methods Peripheral blood mononuclear cells (PBMCs) were isolated from healthy individuals and in which RNA was extracted and reverse-transcripted into cDNA.? light chain genes and Fd fragments of human IgG and IgM were amplified by PCR with designed primers and identified by gel analysis and DNA fingerprinting.Results All the intent immunoglobulin genes were successfully amplified and the obtained products were identified correct and with very good diversity.Conclusion It is feasible to amplify human diversified immunoglobulin genes from PBMCs by RT-PCR using properly designed primers.This method will be useful in the studies of human antibodies,correlative immunological molecules and autoimmune diseases.
