Experimental study of the effect of Angelica polysaccharide on proliferation and differentiation of human CFU-GM and HL-60 cells
- VernacularTitle:当归多糖对人粒单系祖细胞和粒系白血病细胞株增殖分化的实验研究
- Author:
Shali WANG
- Publication Type:Journal Article
- Keywords:
Angelica polysaccharide;
CFU-GM;
HL-60;
Human
- From:
Journal of Chongqing Medical University
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study modern biological mechanism for hematonic and antitumor of Angelica Sinensis (oliv) Diels.Methods:By using the techniques of hematopoietic cells culture in vitro,morphological observation,flow cytometry and immunocytochemistry,the effect of Angelica polysaccharide (APS) on proliferation and differentiation of human CFU-GM and promyelocytic leukemia cell line (HL-60) was studied.Results:APS could markedly promote the colony formation of CFU-GM and could significantly inhibit the proliferation of HL-60 in vitro.The inhibitory effect is concerned with the concentration of APS.APS may prevent HL-60 from the Go phase entering the S/G2+M phase,thus prohibit the synthesis of DNA.APS may also promote HL-60 entering the process of apoptosis.Conclusion:APS may not only promote normal hematopoiesis,but also inhibit the proliferation of leukemia cell and it can be a natural inducer for therapy of malignant tumor.
