The impact of rh-LIF on HL-60 cell line
- VernacularTitle:重组人白血病抑制因子对HL-60细胞的作用及机制研究
- Author:
Bing DU
- Publication Type:Journal Article
- Keywords:
rh-LIF;
Differentiation;
Growth inhibition
- From:
Journal of Chongqing Medical University
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect and mechanism of rh-LIF on proliferation and differentiation of HL-60 cell line.Methods:HL-60 cells were treated with different dosage of rh-LIF for 3 to 6 days,cell cycle was analysed by flow cytometry,expression of P53 and P21 protein were observed by immunohistochemistry and P53 mRNA were assayed by in situ hybridization.Results:HL-60 cell line could differentiate into monocyte and macrophage cell line in the presence of rh-LIF.The proliferation of HL-60 cells were inhibited in the presence of rh-LIF,the cells stagnated at G 1 stage,while expression of P53,P21 protein and P53 mRNA were increased obviously.Conclusion:The effects of rh-LIF on the proliferation and differentiation of HL-60 cells may be associated with the increase of P53,P21 protein and P53mRNA.
