The expression of P-gp during the MDR1 gene transferring into the hematopoietic cells of the Balb/c mouse bone marrow
- VernacularTitle:荷瘤小鼠多药耐药(MDR1)基因转染后P-gp体内表达的研究
- Author:
Chunbao GUO
- Publication Type:Journal Article
- Keywords:
Gene therapy;
Multidrug resistance;
Doxorubicin;
P-gp;
Marrow transplant
- From:
Journal of Chongqing Medical University
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:The study was to improve the protection effect of the bone marrow during the chemotherapy of earlier hepatocarcinoma Balb/C mice,to observe the expression of P-gp in tumor tissues and important organs and to examine the influence of MDR1 gene on Balb/C mouse. Methods:MDR1 gene was transferred into hematopoietic cells of murine bone marrow by the mediation of retrovirus vecter and transplanted into the Balb/c mouse with hepatocarcinoma exposure with 60 Co-? ray in advance.The function and the expression of MDR1 gene in hematopoietic cells,and tumor tissue was detected by RT-PCR method and IC method in vitro.Results:There was no P-gp expression in the two control groups,but that of the different dose experiment group was higher than that of control groups obviously ( P
